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Bio-Ion I

Natural Antibacterial Agent


Bio-Ion: A Natural Alternative:
A number of treatments including chemicals such as chlorine, and irradiation “electronic pasteurization” have been used to control bacterial growth on raw poultry products. However, consumer fears about use of chemicals, and meat irradiation have resulted in the need for alternative treatments.
Bio-Ion is a unique all natural formula combining mineral ions and plant extracts which can be used on raw poultry to control the growth of bacterial populations. Bio-Ion is available in liquid format and can be sprayed on raw poultry, or raw poultry may be dipped into Bio-Ion.
Bio-Ion 1 is organically prepared based on ion salts, using carefully selected proportions to maximize their anti-microbial efficiency through the action of denaturing proteins which weakens the micro-organism, and/or de-polarizing the cell membrane which enhances the ability to penetrate the micro-organism, becoming toxic to the micro-organism and affecting microbial synthesis of DNA and altering its metabolic pathway, causing the cells to lyse and cell death.

Laboratory Tests: 
Nature in Motion ran a series of tests on different types of poultry. Geese, Ducks and chickens.
The majority was done using geese.Short term and long term studies were performed.
Most of the Bio-Ion was added after chilling, however on the last experiment Nature in Motion compared Pre-Chill to Post-Chill and found Bio-Ion was more effective with Pre-Chill.

Sample Collection and Handling:
A carcass with no parts trimmed or missing was randomly selected under the supervision of Dale Donaldson of Ever Spring Farms.
Sterile technique was carefully observed during sample collection and packaging. Each carcass was selected after chilling, at the end of the drip line or at the last readily accessible point prior to cut-up or packaging. Samples were double-bagged and shipped in precooled insulated containers with frozen jell packs and ice sufficient to maintain carcass temperature above freezing but below 10oC during transit.
Samples were to be processed at the laboratory by the day after collection.
Laboratory Procedures:

Geese
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Both carcasses were taken out of their bags and tested for initial bacteria using Easicult tests.
Both were tested at the same location on the carcass. The Easicult test showed they both had equal amounts of bacteria. TNTC (Too Numerous To Count)
Laboratory Procedures:
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Carcass treated with Bio-Ion

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Carcass left untreated as control

One carcass was then put into a new bag aseptically with 1L of 50% solution of Bio-Ion and shaken for two minutes.
Both carcasses were then left to sit for 15 minutes at room temperature, rebagged and put in the refrigerator overnight.
Laboratory Procedures:
The next day each carcass was shaken for two minutes within a sterile bag containing 600 ml of Peptone.
The rinse fluid was then sampled and plated for the recovery of bacteria.
The laboratory used the Dilution plate method.
Pour plates using a variety of Agars for comparison, with the main one being MacConkey Agar.
Easicult tests were also used.
Bio-Ion Results:

Geese
Photo 1: Left is carcass treated with Bio-Ion and Right is the control Carcass (no treatment).
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Photo 1: Left is carcass treated with Bio-Ion and Right is the control Carcass (no treatment).
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Bio-Ion:

Fantastic Results
There was a large difference between the Control and Bio-Ion Treated Poultry. Bio-Ion was always around 1-6 X 10^1 CFU, and Control was always around 3-6X10^3 CFU’s. (All plates are 24 hour culture plates.) Agars used Brilliant Blue, XLD, MacConkey and Hektoen.

Control

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Bio-Ion

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More Great Results
In both photos:
Left: Control Goose Carcass without treatment, TNTC CFU’s.
Right: Goose carcass treated with Bio-Ion 2 CFU’s
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Bio-Ion I - A Natural Intelligent Antibacterial Agent
Bio-Ion I: Antibacterial – Final Summary
This concludes the report of the bacteriological results of the Bio-Ion I to date. This study was conducted by Nature in Motion’s Research Laboratory Division. Liquid cultures were made in triplicate using Bio-Ion combined individually with Escherichia coli (E. coli), Salmonella typhimurium ( S. typhimurium), Staphylococcus aureus (S. aureus), Listeria monocytes (L. monocytes), and Bacillus subtilis(B. subtilis). Liquid culture controls of each bacteria individually were done in triplicate for comparison. Samples were collected at 24 hours and 48 hours from each of the samples and the Optical Densities read using a Spectrophotometer at the wavelength of 550nm. The individual samples were then combined to form a composite sample. The composite sample was analyzed and plated for recovery of E. coli, S. typhimurium, S. aureus. L. monocytes, and B. subtilis . Supporting data used in the interpretations concluded that 100% of the Bio-Ion liquid cultures sampled had 0 Colony Forming Units (CFU’s) of E. coli, S. typhimurium, S. aureus. L. monocytes, and B. subtilis. All of the controls had high numbers of CFU’s with E. coli, S. typhimurium, S. aureus. L. monocytes, and B. subtilis. More bacteriological studies are in the process.